21-07-08(23:40:02)
Authors:
Bakay RAE,Smith AP,Kelly LP,Newman MB
Institutions:
Departments of Neurosurgery1, Rush University Medical Center, 1735 W. Harrison St. Cohn Research Building, Suite 406, Chicago, IL 60612.
Title of abstract : Therapeutic Use of Human Embryonic Stem Cells (hESC) and Human Umbilical Cord Blood (hUCB) Stem Cell in Parkinson’s Disease Model.
Abstract text:
The present study was designed to determine the potential of neural induced and non-induced stem cells of different origins to repair, replace, or induce endogenous regeneration of the damaged tissue and loss of DA in the striatum in adult rats lesioned with the DA neurotoxin 6-hydroxydopamine (6-OHDA), a model of Parkinson’s disease. We investigated viability and potential of 1) hESC progenitor cells, 2) neural induced hESC, 3) the mononuclear fraction of hUCB, and 4) CD133 stem cells a subset of hUCB, in the 6-OHDA lesioned rats.
The hESC cells were cultured in serum-free defined media for 7 to 21 days in vitro (DIV). The hUCB cells and the CD133 stem cells were cultured in serum free defined media for hematopoietic stem cells for 5 to 21 DIV. A total of 1 X 106 cells, were transplanted into the striatum of rats 4 weeks after receiving 6-OHDA in middle forebrain bundle or were sham lesioned. Behavioral testing was preformed pre-lesion, post-lesion (to met criteria), and post-transplant. After transplantation, behavioral testing was performed every other week until brains were taken at 2 months and processed for immunohistochemistry.
Result of behavioral rotation data for the hESC non-induced rats showed 29% recovery, whereas rats in the hESC neural induced condition showed 59% recovery. Both hUCB conditions showed almost complete recovery in the rotational behavior test. Analysis with dependent measures which included, number of grafted cells, cell counts for DA neurons in substantia nigra, and inflammatory markers will be presented.
