INTR10

Authors:

Rath A 1, Papazoglou A 1, Hackl C 1, Klein A 1, Nikkhah G 1

Institutions:

1 Department of Stereotactic Neurosurgery, Laboratory of Molecular Neurosurgery, Neurocentre, University Hospital Freiburg
Breisacher Str. 64
79106 Freiburg
Germany

Title of abstract : Effects of Two-Step-Grafting on the survival of embryonal (E14) mesencephalic dopaminergic neurons and on the striatal reinnervation in a rat model of Parkinson’s disease (PD)

Abstract text:

In a rat model of Parkinson’s disease (PD), unilateral injections of 6-hydroxydopamine into the medial forebrain bundle leads to a depletion of dopamine (DA) within the relevant striatum. After the lesion, primary cell suspensions, derived from ventral mesencephalon of E14 rat embryos, are transplanted into the lesioned striatum.
In a former study by our group, we established a new protocol based on two transplantation (TX) time points in order to improve the survival of the grafted cells. Rats received half of the standard amount of VM cells during the first transplantation and the other half during the second graft after defined time points (3, 5, 7 or 9 days). To distinguish between the two grafts, cells were labeled either with PKH 26 or with PKH 67 staining assay. Morphological analysis showed a significant higher survival of DAergic cells and a tendency to higher graft-volume and fiber-density in the group of 5 days compared to a standard group.
In this study, in order to examine a possible influence of the cell labeling protocol on the transplanted cells, rats received a standard TX, stained cells transplanted by the standard protocol or a two-step-grafting TX (second TX after 5 days, non-treated cells).
Morphological analysis shows a tendency to higher cell survival, graft volume and fiber density in the two-step-grafting group compared to the standard group. Groups which received treated cells were similar to the standard group.
Taken together, the two-step-grafting strategy offers an interesting model to study graft-host-interactions in PD and we will concentrate on analyzing the molecular interactions between the two grafts.

This entry was posted on Wednesday, July 16th, 2008 at 08:38 and is filed under PD: models and applications, all. You can follow any responses to this entry through the RSS 2.0 feed. Responses are currently closed, but you can trackback from your own site.