INTR10

Authors:

Krause M 1, Papazoglou A 1, Ganser C 1, Nikkhah G 1, Kobayashi E 2

Institutions:

1 Stereotactic Neurosurgery, Laboratory of Molecular Neurosurgery, Neurocentre, University Hospital Freiburg, Breisacher Str. 64, 79106 Freiburg, Germany
2 Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan

Title of abstract : Green Fluorescent Protein (GFP) transgenic rats: A new tool for neural transplantation

Abstract text:

Studying the graft survival can advance the stem cell transplantation protocols for the patient benefit. However, common cell labelling methods cannot provide stable labelled cells that can be detected after long-term transplantation period. GFP transgenic rats (Inoue et al, BBRC 329 (2005)) express GFP ubiquitously providing an ideal donor source. The aim of this project is to investigate the potential of these rats to become a useful tool in the field of neuroscience.
In this study, we used the 6-OHDA rat model of Parkinson disease based on unilateral injections of 6-hydroxydopamine into the medial forebrain bundle. Primary cell suspensions derived from dissociated ventral mesencephala of E14 GFP-Lewis rat embryos were transplanted into the striatum of lesioned Sprague Dawley rats. Considering that donor and the recipient belong to two different rat strains, special focus was set on graft survival in correlation with immunosuppression and GFP-expression. Transplanted animals were divided into two groups, one with and one without immunosuppression. Animals of each group were sacrificed 2, 4, 10 and 15 weeks after transplantation. Lesion and transplantation effects were evaluated with drug-induced rotations 6 weeks after lesion and 2, 4, 10 and 15 weeks after transplantation.
All experimental groups showed a significant compensation after transplantation on rotation behaviour. Morphological analysis revealed that graft survival showed no significant difference between immunosuppressed and non-immunosuppressed animals. In addition, GFP-expression remained stable in most cell compartments of the graft.
Taken together, GFP-rats serve as an excellent tool for studying neural stem plasticity in the transplantation paradigm.

This entry was posted on Wednesday, July 16th, 2008 at 08:37 and is filed under PD: models and applications, all. You can follow any responses to this entry through the RSS 2.0 feed. Responses are currently closed, but you can trackback from your own site.