INTR10

Authors:

J. Scheibe, A. Focke1, S.C. Schwarz, J. Schwarz

Institutions:

Department of Neurology, University of Leipzig

Title of abstract : Transplantation efficacy of different cultivated and VSOP labelled human neural progenitor cells (hNPCs) in an animal model for Parkinson’s disease (PD)

Abstract text:

Mesencephalic neural precursor cells are a very promising source for therapeutic approaches regarding cell replacement therapy for Parkinson’s disease (PD). Until now it is possible to expand the cells in vitro. In this study two different cell lines have been proliferated, differentiated and VSOP labelled to examine integration and migration properties after transplantation into 6-OHDA unilateral lesioned rats, a useful model for PD. Data was collected via behaviour tests, MR-Imaging and post mortem Immunhistochemnistry. Transplantation of differentiated cells led over a three months time period to a brief advance in rat behavior. Animal of one group showed a higher amount of TH-positive cells. Asuming that integration of cells is partially depending on proliferation durability.
Labeling and in vivo tracking of hmNPCs with VSOPs in a sufficient amount was also possible. The amount of Prussian Blue positive cells in sham rats is less compared to labelled rats. Additionally, the staining in sham rats is strictly localized in the needle tract. We assume that this investigation is due to hem iron of bleeding under transplantation procedure. Therefore, VSOPs are a suitable tracking agent
for MR imaging and histologic issues of transplanted human neural precursor cells.

This entry was posted on Wednesday, July 16th, 2008 at 10:00 and is filed under Stem cells, all. You can follow any responses to this entry through the RSS 2.0 feed. Responses are currently closed, but you can trackback from your own site.