15-07-08(14:09:19)
Authors:
Takao Yasuhara, Satoshi Kuramoto, Takashi Agari, Yasuyuki Miyoshi, Isao Date
Institutions:
Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Title of abstract : Erythropoietin delays initiation of rapid amygdala kindling in the rat
Abstract text:
Erythropoietin (EPO), recognized for its central role in erythropoiesis also mediates neuroprotection. In this study, we examined how EPO affected kindling epileptogenesis. Adult male Wistar rats were used. One bipolar electrode was implanted in the right amygdaloid complex. After surgery, the animals were allowed a week for recovery. The rapid amygdala kindling protocol employed kindling stimulations (10 s, 60 Hz trains of 1 msec biphasic square wave pulses at 400 μA) administered via the bipolar electrode up to 24 times per day, with a 15-min interstimulation-interval. Evoked seizures were graded using the Racine classification. Kindling was stopped when rats reached a fully kindled state (defined as 5 ClassV-seizures) or after 120 stimulations. Continuous infusion into the right lateral ventricle with EPO (100U/day for seven days) or rat serum albumin was started right before the first stimulation. Rats were sacrificed at 7days after final stimulation. Consequently, more stimulations were required in the EPO treated group than control group. That is to say, EPO delayed the progression of kindling. Immunohistochemical analysis by staining for NeuN revealed a reduced loss of CA1 pyramidal neurons in the EPO treated group. Moreover, the number of BrdU/GFAP positive cells decreased in the CA1 and hilus of the EPO treated group. These results suggest that EPO inhibits hippocampal neuronal loss and microglial activation after status epilepticus and exerts anti-epileptogenic properties on seizures originating within amygdala.
