15-07-08(13:34:33)
Authors:
Jiang W.1, Döbrössy M.1, Papazoglou A.1, Büchele F.1, Diaconu O.1, Garcia J.1, Nikkhah G.1
Institutions:
1 Stereotactic Neurosurgery, Laboratory of Molecular Neurosurgery, Neurocentre, University Hospital Freiburg, Freiburg, Germany
Title of abstract : Micro-transplantation approach in a quinolinic acid induced rodent model of Huntington’s disease
Abstract text:
Neural transplantation has been demonstrated to be an optional treatment for Huntington’s disease (HD). In this study, we investigated the different neuroanatomical and behavioural effects between single- (ST) and multi-tracts (MT) transplants performed in a HD rodent model.
42 female Sprague Dawley rats received unilateral quinolinic acid injections into the left striatum. 10 days after the quinolinic acid lesion the animals were transplanted with a rich GABAergic progenitors single cell suspension (480,000 cells/animal) derived from the striatum of E15 GFP rat embryos. Grafting procedure included 1 tract/2 deposits (ST) or 9 tracts/18 deposits (MT) transplants using a 2µl Hamilton’s syringe with capillary. Animals were trained prior to lesion and 4, 8, 12 weeks post transplantation in spontaneous behaviour, skilled forelimb use and apomorphine rotation. Animals were perfused and brain tissue was processed for immunohistochemistry. Graft volume and cell survival will be assessed by stereology.
After lesion all the lesioned rats showed significant functional deficits in all the behavioural tests and apomorphine-induced rotation test. One and two months after transplantation ST and MT groups did not show any significant functional recovery in the stair case as well as in the rotation test. Morphological graft analysis will be presented at the conference.
Skilled forelimb can be used as a sensitive method to evaluate the quinonilic acid induced lesion. Evidence will be provided at the conference if the micro transplantation approach with MT transplants can offer significant advantages for HD as was shown for Parkinson’s disease.
