INTR10

Authors:

Mazurová Y 1, Guncová I 1, Rudolf E 2, Látr I 3

Institutions:

1Dept. of Histology and Embryology and 2Dept. of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Králové, 3Neurosurgery Clinic, Faculty Hospital, Hradec Králové - Czech Republic

Title of abstract : Huntington’s disease model: Compartmentalization of neurogenic niche in response to neurodegenerative process within the striatum.

Abstract text:

In the subependymal zone (SEZ), GFAP-positive astrocytes have been identified basic cellular elements of neurogenic niche – the niche cells, as well as the neural stem cells. The neurodegenerative process in the striatum in a rat model of Huntington’s disease (HD) initiates immediate intensive cell proliferation and differentiation, particularly of the GFAP+ astrocytes, but in HD patients chronic pathological process develops. Therefore, we were interested in a structure of the SEZ at 7 days and 6 months after QA lesion. We described the compartmentalization of SEZ according to the character and intensity of response to striatal damage, to the rostral part of the SEZ (R-SEZ), related to the rostral migratory stream and olfactory bulb, and the lateral part (L-SEZ), opposite the degenerated striatum. Our results show some new facts: (1) the astrocyte-like cells in adult neurogenic niche activated by neurodegenerative process within the striatum reveal a wide plasticity (the comparison of two compartments of the SEZ), (2) GFAP+ astrocytes of adult SEZ are quiescent NSCs which retain some features of embryonic stem cells - the reexpression of nestin and particularly of vimentin which is typical also for radial glia - but their proliferation and differentiation are limited by the microenvironment of adult (secondary) neurogenic niche, and (3) although the intensity of cellular response of the SEZ to the striatal lesion decreases during the time, characteristic changes in a structure of neurogenic niche were found during whole watched period.
Supported by the Research project (MSM0021620820) of the Ministry of Education of CR.

This entry was posted on Thursday, June 26th, 2008 at 16:01 and is filed under HD: clinical applications, all. You can follow any responses to this entry through the RSS 2.0 feed. Responses are currently closed, but you can trackback from your own site.